Type I IFN upregulates IL-10 production by Th17 cells.

<p>(<b>A</b>) and (<b>B</b>) Naïve CD4 T cells were cultured in Th17 culture conditions in the presence of purified IFNα or IFNβ (500 U/ml) for 72 hours. Production of IL-17 and IL-10 by CD4 T cells was determined by ELISA. (<b>C</b>) IL-10^−/− naive CD4 T cells were cultured in Th17 culture condition with IFNβ for 72 hours, the concentration of IL-17 in the supernatants was measured by ELISA. Results are reported as mean±SD of triplicate samples from one representative experiment. Data are representative of five (A, B) and three (C) experiments with similar results.