<p>Naïve CD4 T cells were cultured in Th17 polarizing conditions as described above in the presence of IFNβ for 72 hours. Expression of IL-17 and IL-10 in CD4 T cells was determined by intracellular cytokine staining. Data are representative of five experiments with similar results.</p
<p>(A) Sorted TCR-transgenic cells were stimulated <i>in vitro</i> with peptide-loaded DCs under cul...
<p>Purified CD4<sup>+</sup> T cells were stimulated with plate-bound anti-CD3 in the presence or the...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
<p>(<b>A</b>). Proliferation of IL-10<sup>+</sup> and IL-17<sup>+</sup> CD4 T cells upon IFNβ treatm...
<p>(<b>A</b>) and (<b>B</b>) Naïve CD4 T cells were cultured in Th17 culture conditions in the prese...
<p>Naïve T cells cultured with or without IFN-β in Th1-polarizing conditions (10 ng/ml IL-12) (A) or...
<p>(<b>A</b>) and (<b>B</b>) CD4 T cells were cultured under the Th17 conditions in the presence of ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>CD4+CD25- T cells were stimulated in the presence of Th17 polarising cytokines for four days and ...
Cells were harvested on day 6 and restimulated with PMA plus ionomycin, followed by intracellular cy...
<p>Highly purified naive CD4<sup>+</sup> T cells from female C57BL/6 mice were incubated for 2 h wit...
<p>Effector/memory T cells were cultured with or without IFN-β for 72 hours. Cytokines were detected...
<p>(a) CD4<sup>+</sup> T cells isolated from wild type (WT) C57BL/6 (B6) mice and IFN-γ knock out (K...
<p>Naïve CD4<sup>+</sup> or CD8<sup>+</sup> cells from AhR<sup>+/−</sup> mice were cultured for 96 h...
<p>(A) Naïve CD4<sup>+</sup> cells were sorted from spleens of OTII animals. Cells were then co-cult...
<p>(A) Sorted TCR-transgenic cells were stimulated <i>in vitro</i> with peptide-loaded DCs under cul...
<p>Purified CD4<sup>+</sup> T cells were stimulated with plate-bound anti-CD3 in the presence or the...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
<p>(<b>A</b>). Proliferation of IL-10<sup>+</sup> and IL-17<sup>+</sup> CD4 T cells upon IFNβ treatm...
<p>(<b>A</b>) and (<b>B</b>) Naïve CD4 T cells were cultured in Th17 culture conditions in the prese...
<p>Naïve T cells cultured with or without IFN-β in Th1-polarizing conditions (10 ng/ml IL-12) (A) or...
<p>(<b>A</b>) and (<b>B</b>) CD4 T cells were cultured under the Th17 conditions in the presence of ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>CD4+CD25- T cells were stimulated in the presence of Th17 polarising cytokines for four days and ...
Cells were harvested on day 6 and restimulated with PMA plus ionomycin, followed by intracellular cy...
<p>Highly purified naive CD4<sup>+</sup> T cells from female C57BL/6 mice were incubated for 2 h wit...
<p>Effector/memory T cells were cultured with or without IFN-β for 72 hours. Cytokines were detected...
<p>(a) CD4<sup>+</sup> T cells isolated from wild type (WT) C57BL/6 (B6) mice and IFN-γ knock out (K...
<p>Naïve CD4<sup>+</sup> or CD8<sup>+</sup> cells from AhR<sup>+/−</sup> mice were cultured for 96 h...
<p>(A) Naïve CD4<sup>+</sup> cells were sorted from spleens of OTII animals. Cells were then co-cult...
<p>(A) Sorted TCR-transgenic cells were stimulated <i>in vitro</i> with peptide-loaded DCs under cul...
<p>Purified CD4<sup>+</sup> T cells were stimulated with plate-bound anti-CD3 in the presence or the...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...