Post-transcriptional regulation of miR-21 expression by PTEN.

<p>(A) Time course of pri- (small dotted line), pre- (large dotted line), or mature miR-21 (single unbroken line) expression in U87MG cells after HA treatment. Expression level of each miRNA was analyzed by qRT-PCR reactions which were normalized to GAPDH for pri- and pre-miR-21, and U6 small nuclear RNA for mature miR-21. (B) Expression level of mature miR-21. U87MG cells pretreated with ActD or overexpressing PTEN were treated with HA for 24 hr. Data represent the mean values of at least three independent experiments performed in triplicate. **P<0.01 and ***P<0.001; n.s., not significant; Student's t test. Error bars indicate s.e.m. PTEN expression and AKT phosphorylation were confirmed by immunoblotting.