EDL Force vs. stimulation frequency relationship assayed <i>in vitro</i>.

<p>Trains of pulses (0.2 msec.) delivered at 1, 30, 50, 80, 100,150 and 300 Hz. <b>A.</b> The absolute isometric force vs. stimulation frequency relationship between WT and CASQ1 null EDLs revealed significantly reduced tension in the CASQ1 nulls between 40–300 Hz. However, EDL muscles from the CASQ1 null mice (7.58±0.43 mg; n = 8) were approximately 29% smaller in mass than those from the WT age matched controls (10.73±0.71 mg; n = 8, t-test, p<0.05). <b>B.</b> Following CSA normalization the force vs. stimulation frequency relationship revealed small but significant differences in the amplitudes of low frequency trains (1 and 20 Hz and 40 Hz) while no differences were seen at any other frequency (ANOVA).