Immunofluorescence analysis of Dab1 (red) and phospho-SFK^Y416 (green) in the developing human retina.

<p>Sections were double-stained with anti-Dab1 and anti-phospho-SFK^Y416 (pSFK) antibodies followed by Alexa 555-conjugated goat anti-rabbit and Alexa 488-conjugated donkey anti-mouse secondary antibodies. Sections were counterstained with the fluorescent dye Hoescht 33258 to label the nuclei. Retinal tissue sections were prepared from human fetal retina at 8 wks gestation (<b>A</b>) and 13 wks gestation (<b>B</b>). The strong staining observed in the RPE is caused by autofluorescence. Abbreviations are: RPE, retinal pigment epithelium; INBL, inner neuroblastic layer; NFL, nerve fiber layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.