Temporal expression of RPA and MSH4 in wild-type and SC-null oocytes.

<p>Oocytes at different stages of meiotic prophase were labeled with antisera to RPA or MSH4 (red). The chromosomal axes were visualized by STAG3 protein labeling (blue). The RPA and MSH4 recombination-related proteins disappear from the chromosomal axes in wild-type oocytes by late pachytene. In SC-null oocytes MSH4 shows a pattern similar to that seen in wild-type oocytes, while RPA foci persist until the diplotene stage. Bar, 10 µm.