Effect of cerebral ischemia-reperfusion on vascular superoxide production.

<p>Basal (A and B) and phorbol-12, 13-dibutyrate (10 µmol/L; PDB: Nox2 activator)-stimulated (C and D) superoxide production by middle cerebral arteries (MCA) as measured by 100 µmol/L L-012-enhanced chemiluminescence. <b>A.</b> Contralateral and ischemic MCA from wild-type mice 24 h after MCA occlusion, and pooled left and right MCA from sham-operated wild-type mice. <b>B.</b> Contralateral and ischemic MCA from Nox2-deficient (Nox2^-/-) mice 24 h after MCAO. <b>C.</b> Contralateral and ischemic MCA from wild-type mice 24 h after MCA occlusion (MCAO), and pooled left and right MCA from sham operated wild-type mice. <b>D.</b> Contralateral and ischemic MCA from Nox2^-/- 24 h after MCAO. All results are expressed as 10³ counts/mg of dry tissue weight and given as mean±SEM (A and C, n = 9 for both groups of MCAO; n = 7 for sham; B and D, n = 6 for both groups). *<i>P</i>&lt;0.05 vs. contralateral MCA (one way ANOVA with a Bonferroni mutiple comparison post hoc test).