Lineage positive (Lin⁺) cells.

<p>Panel A showed the representative Confocal images of Lin⁺ antibody staining (red-APC) on freshly isolated cells as well as plated cells at P0, P6, and P10 with (+) and/or without (−) c-kit MACS and/or Lin-dep (Panel A, top row-with c-kit and Lin-dep; middle row with c-kit, but without Lin-dep; and bottom row without c-kit and Lin-dep). Cell nuclei were stained in blue-DAPI, and Scale bars = 20 µm. MACS were able to be performed at P1 to P2. Human bone marrow (m to o) was used as a positive control for Lin markers (red-APC). Panel B showed the Mean±SEM data from ICC. ***: <i>p</i><0.001 (P0, P6, P10 and human bone marrow) compared with control group (Fresh isolated cells). Panel C displayed the representative data of Flow Cytometry with double staining of c-kit-FITC (x-axis) and Lin-APC (y-axis) in CSCs before (upper) and after (middle) Lin-dep. Human bone marrow was used as positive control (lower). Panel D showed the Mean±SEM) data of Flow Cytometry from three patients. ***: <i>p</i><0.001 and ns: no significance; both were compared with control level (Before Lin-dep).