Different neuronal phenotypes developed from radial glia-like cells.

<p>Neurons with different neurotransmitter-phenotypes developed from cloned populations of RGl-cells. Genes indicating noradrenergic (<i>Dbh</i>), serotonergic (<i>Tph2</i>) and cholinergic (<i>Chat</i>) neurotransmitter phenotypes were not expressed in neuron-rich cultures of any RGl-cells regardless of fetal or adult origin (<b>a</b>). GABA- and VGAT-immunopositive GABAergic (<b>b</b>, <b>c</b> respectively) and VGlut2-immunopositive glutamatergic (<b>d</b>) neurons developed in embryo-derived clones upon EGF-withdrawal (cells from clone C4 are shown). All adult-derived clones generated GABAergic neurons. Hippocampus- derived HC_A cells produced <i>VGlut1</i>-expressing (<b>a</b>) neurons, and all SVZ-clones gave rise to tyrosine-hydroxylase (TH)-positive (<b>e, e’</b>) but <i>dbh</i>-negative (<b>a</b>), neurons (<b>e</b>, <b>e’</b>: neurons from SVZ_M clone are shown). The pictures were taken on the 11^th day after EGF-withdrawal.