Interaction of LEC and pancreatic macrophages.

<p>(A) CX3CR1^hi macrophages, LYVE-1⁺ macrophages, CD11b⁻-lymphocytes and LEC were sorted from pancreatic single cell suspensions of CX3CR1^GFP/+ mice. Co-cultured CX3CR1^hi macrophages-CFSE, LYVE-1⁺ macrophages-eFlour670, or lymphocytes-CFSE with/without LEC on Matrigel for 5 days. Scale bars: 60 µm. 100× magnification. (B) Immunofluorescent staining of CD68 and LYVE-1 in pancreas from C57BL/6 mice. Scale bars: 160 µm. 50× magnification. (C) Sorted CX3CR1^hi macrophages labeled with CFSE and co-cultured with LEC (left upper panel) or without LEC (left lower panel) for 5 days, and stained for CD11b (red) and LYVE-1 (yellow). Scale bars: 10 µm. 630× magnification. Right panel, quantitative analysis, total cells from 5 fields (1344×1024 pixels) were counted. All data representative of 2 to 4 separate experiments.