Detection of siRNA in <i>Phytophthora parasitica</i> re-isolated from transgenic plants producing <i>GFP</i> siRNAs or <i>PnPMA1</i> siRNAs.

<p>dsPMA1 and dsGFP <i>Arabidopsis</i>, <i>P. parasitica</i> re-isolated from transgenic plants expressing <i>PnPMA1</i> dsRNA and <i>GFP</i> dsRNA, respectively; WT, <i>P. parasitica</i> isolated from diseased wild-type <i>A. thaliana</i>; lanes 1–3, <i>P. parasitica</i> cultures five days after being isolated from three independent transgenic <i>A. thaliana</i> lines producing <i>GFP</i> siRNAs or <i>PnPMA1</i> siRNAs; P1, A <i>P. parasitica</i> transformant accumulated <i>PnPMA1</i> siRNAs as a positive control for <i>PnPMA1</i> siRNA detection; P2, A transgenic <i>A. thaliana</i> line accumulated <i>GFP</i> siRNAs as a positive control for <i>GFP</i> siRNA detection; M, RNA size markers, from bottom up are 21 and 24 nt RNAs, respectively.