Plot of observed rate constants of various intermediates that formed upon mixing of LPO-Fe(III) or LPO-Fe(II)-O₂ against increasing concentration of HOCl.

<p>The observed rates of LPO-Fe(III)-OCl formation monitored at 432 nm (Panel A); Compound II accumulation (Panel B); and Compound II decay(Panel C); as for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027641#pone-0027641-g002" target="_blank">Fig. 2</a>, were plotted as a function of HOCl concentration. In Panel C, the close triangle represent kinetic parameters for Compound II decay to LPO-Fe(III), while the close circles represent kinetic parameters for Compound II destruction. The inset shows the rate of heme destruction, the curve was extrapolated to zero. Panel C is the plots of the observed rates for the LPO Compound III heme destruction versus HOCl concentration, monitored at 424 nm. The data are representative of three separate experiments.