Effect of Tm-TNF on <i>M. tuberculosis</i> replication during reactivation.

<p>WT mice (black circles), TNF^−/− mice (white diamonds) and Tm-TNF mice (white squares) were treated for 6 weeks with 25 mg/Kg INH-RIF in drinking water subsequent to 3 weeks aerosol infection with 100–200 CFU's of <i>M. tuberculosis</i> H37Rv. (A) Body weights were recorded throughout the course of the infection period and bacterial burdens in lungs (B) and spleens (C) were enumerated at time points indicated. Data are representative of two experiments and data points are expressed as the mean ± SD of 5 mice/group (for CFUs). The body weight study consisted of between 6–16 mice/group where the data represents weights for remaining mice in each group. The red “T” in the figure corresponds to the drug treatment phase. Significant differences (*<i>p</i><0.05; **<i>p</i><0.01) were determined by Student's <i>t</i> test for comparisons between two groups and ANOVA for comparisons between three groups.