RhCMVIL-10-NAbs generated against RhCMVIL-10 M1 and M2 do not cross react with cellular RhIL-10.

<p>Plasma from animals (A1–A6) pre- (pre-Imm, week 0, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028127#pone-0028127-g007" target="_blank">Figure 7</a>), and post-immunization (week 19 plasma sample, 1 week after the second protein boost, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028127#pone-0028127-g007" target="_blank">Figure 7</a>), was tested for its ability to neutralize cellular RhIL-10 (grey bars) or RhCMVIL-10 (black bars) bioactivity, where bioactivity was measured as suppression of LPS-induced IL-12 production in rhesus PBMC. The data show the biological activity of RhIL-10 is not neutralized by plasma from animals immunized with RhCMVIL-10 M1 or M2. However, RhCMVIL-10 biological activity, except for A6, is almost completely neutralized by the same plasma sample. Percent (%) neutralization is calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028127#pone-0028127-g007" target="_blank">Figure 7</a> and in the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028127#s4" target="_blank">Materials and methods</a>” section.