Laser-induced choroidal neovascularization in wild-type and <i>C1qtnf5</i> Ser163 Arg knock-in mice.

<p><b>A</b>, Fundus images from the early and late phase of <i>in vivo</i> fundus fluorescein angiography at 1 and 2 weeks after photocoagulation/laser induced choroidal neovascularization (CNV) in 15–16 month old wild-type and <i>C1qtnf5</i> KI mice. Wt: wild-type mice, Het: heterozygous KI mice, Mut: homozygous KI mice. <b>B</b>, The microglia in laser lesions on RPE flat mounts resulting from CNV laser treated <i>C1qtnf5</i> KI mice after 2 weeks were labelled with anti-Iba1 (green) and co-labelled with anti-lectin (blood vessels) antibodies. Scale bar, 250 µm. <b>C</b>,<b>D</b>, Quantitative analysis of area of hyperfluorescence in the fundus images from 1 week old animals (<b>C</b>) and 2 week old animals (<b>D</b>) as a measure for CNV lesion size. No significant difference between the hyperfluorescent areas were observed at 1 week (Kruskal-Wallis with Dunn's mutliple comparison test, p = 0.911) or 2 weeks (Kruskal-Wallis with Dunn's mutliple comparison test, p = 0.638). The number of animals for both time points were n (wt) = 4, n (het) = 12, n(mut) = 10.