Single Point Highest Inhibition Achievable assay (SPHIAa) for LTP IgE inhibition.

<p>IgE values on graphs are reported as percent inhibition. IgE values for each serum in each graph representation are given in brackets. The three inhibitors are indicated in the X axis, whereas on top of each graph the LTP immobilized on the microarray whose IgE binding has been inhibited is reported. Statistical evaluations have been applied to the three series using the Kruskal-Wallis One-Way ANOVA by Ranks , followed by the <i>post hoc</i> test of Nemenyi-Damico-Wolfe-Dunn comparing paired series. Statistics are as follows: <b>Panel A - nAct c 10</b>: nAct c 10 Median = 100%; Range 83.91–100%; nAct d 10 Median = 100%; Range 97.92–100%; nPru p 3 Median = 100%; Range 78.03–100%; Kruskal-Wallis = n.s.s.; <b>Panel B - nAct d 10</b>: nAct c 10 Median = 100%; Range 84.27–100%; nAct d 10 Median = 100%; Range 98.67–100%; nPru p 3 Median = 100%; Range 80.42–100%; Kruskal-Wallis = n.s.s.; <b>Panel C - rAra h 9</b>: nAct c 10 Median = 79.62%; Range 50.18– 97.85%; nAct d 10 Median = 85.39%; Range 51.00–100%; nPru p 3 Median = 100%; Range 88.05–100%; Kruskal-Wallis p = 0.00061; nAct c 10 <i>vs</i> nAct d 10 = n.n.s.; nAct c 10 <i>vs</i> nPru p 3 p = 0.00019; nAct c 10 <i>vs</i> nPru p 3 p = 0.0055; <b>Panel D - nArt v 3</b>: nAct c 10 Median = 75.45%; Range 0–100%; nAct d 10 Median = 79.04%; Range 0–100%; nPru p 3 Median = 100%; Range 90.80–100%; Kruskal-Wallis p = 0.0012; nAct c 10 <i>vs</i> nAct d 10 = n.n.s.; nAct c 10 <i>vs</i> nPru p 3 p = 0.00078; nAct c 10 <i>vs</i> nPru p 3 p = 0.0083; <b>Panel E - rCor a 8</b>: nAct c 10 Median = 95.83%; Range 32.44–100%; nAct d 10 Median = 85.79%; Range 0–100%; nPru p 3 Median = 100%; Range 80.01–100%; Kruskal-Wallis p = n.n.s.; <b>Panel F - nMor n 3</b>: nAct c 10 Median = 63.71%; Range 27.97–95.84%; nAct d 10 Median = 67.06%; Range 43.65–97.31%; nPru p 3 Median = 98.99%; Range 85.11–100%; Kruskal-Wallis p = 0.00031; nAct c 10 vs nAct d 10 = n.n.s.; nAct c 10 vs nPru p 3 p = 0.00035; nAct c 10 vs nPru p 3 p = 0.0011; <b>Panel G - nPru p 3</b>: nAct c 10 Median = 50.21%; Range 18.25–95.94%; nAct d 10 Median = 60.55%; Range 39.55–98.11%; nPru p 3 Median = 100%; Range 97.44–100%; Kruskal-Wallis p<0.0001; nAct c 10 vs nAct d 10 = n.n.s.; nAct c 10 vs nPru p 3 p<0.0001; nAct c 10 vs nPru p 3 p = 0.0003.