Levels of P-Smad1/5 correlate with the strength of NCPC phenotype in <i>smad5</i> morphant embryos.

<p>(A) P-Smad1/5 levels in <i>smad5</i> morphant embryos. Embryos injected with increasing higher concentrations of <i>smad5</i> MOs show decreasing P-Smad1/5 levels relative to uninjected controls. Actin was used as a loading control. (B, C) Expression of <i>foxd3</i> in embryos injected with increasing doses of <i>smad5</i> MOs. Injection of a low dose of <i>smad5</i> MOs (3 ng) leads to WT, “<i>snh</i>” and “<i>sbn</i>” phenotypes, whereas injection of higher doses of <i>smad5</i> MOs (5 ng, 6 ng) lead to “<i>sbn</i>” and “<i>swr</i>” phenotypes. The embryos used for in situ hybridization of <i>foxd3</i> in Fig. 4B are from the same batch of injected embryos used for Western blotting in Fig. 4A.