Antibody blocking ELISpot.

<p>FSP-specific CTLs were stimulated against T2 cells either loaded with FSP31 or irrelevant peptide (P68). HLA-specificity was examined by blocking MHC-I on the target cell side (HLA-A2 and pan-MHC-I). Inhibition of IFN-γ release was confirmed by blocking CD3, CD8 and CD28 on the T cell side. The number of IFN-γ releasing T cells is given. Results are displayed as the mean and standard deviation from analysis performed in triplicate.