Effects of amine-receptor antagonists on cAMP responses mediated via <i>Am</i>DOP2 and <i>Am</i>OA1 receptors.

<p>HEK293 cells were co-transfected with expression constructs for either the <i>Am</i>DOP2 receptor (blue symbols) or <i>Am</i>OA1 receptor (red symbols) and a CRE-luciferase reporter construct. Cells were treated with 1 µM agonist (DA or OA respectively) or 1 µM agonist and either <i>cis</i>-(Z)-flupentixol (A), spiperone (B), mianserin (C) or epinastine (D), at a range of concentrations indicated in the figure. Due to evidence of significant cell toxicity, <i>cis</i>-(Z)-flupentixol was not tested at a concentration higher than 10 µM. Data are normalized to the response observed in cells treated with agonist alone (not shown), and are the result of two independent experiments within which, each treatment was tested twice. Error bars (estimated SEM) are included to provide an indication of consistency between experiments. Dose response curves were determined by non-linear regression using GraphPad Prism software for Macintosh version 5.0b.