KIAA0101 mRNA and protein expressions were decreased with siRNA knockdown in the NCI-H295R adrenocortical carcinoma cell line.

<p><b>A</b>) H295R cells were transfected with KIAA0101 siRNA and negative control and analyzed for KIAA0101 mRNA expression after 48 hours of treatment. Columns represent KIAA0101 mRNA expression percentage relative to negative control ± standard deviation of four experiments. <b>B</b>) Representative Western blot demonstrating protein expression of KIAA0101 after 6 days of siRNA and negative control. <b>C</b>) Relative intensity of western blot using Image J Software. Columns indicate ratio of KIAA0101 and GAPDH intensity measurements. NC40 = Negative control at 40 nM; SI40 = siRNA at 40 nM; NC80 = Negative control at 80 nM; SI80 = siRNA at 80 nM.