Translocation of mitochondrial apoptotic factors is induced by FE.

<p>(A) Time-dependent release of AIF from mitochondria into the cytosol was measured by western blotting analysis. (B) FE induces translocation of AIF from the mitochondria to the nucleus. MCF-7 cells were treated with 820 µg/mL FE for 96 h. After incubation with 100 nM MitoTracker Red (red fluorescence), the cells were fixed and permeabilized followed by immunostaining with an antibody specific for AIF (green fluorescence) and counterstaining with Hoechst 33342 (blue fluorescence) to visualize nuclear morphology. (C) Time-dependent release of cytochrome <i>c</i> from mitochondria into the cytosol was measured by western blotting analysis. Similar results were obtained from 3 independent experiments. Each image shown is representative of 20 random fields observed.