Treatment with Ashwagandha i-Extract/withanone and protection against scopolamine-induced DNA damage and oxidative stress.

<p>Level of expression of DNA damage marker (γH2AX) (A) and oxidative stress marker (reactive oxygen species, ROS) (B) in C6 cells treated with either scopolamine or scopolamine and i-Extract/withaferin A/withanone as detected by immunocytochemistry with specific antibodies and as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027265#s4" target="_blank">material and methods</a> are shown. Quantitation of γH2AX (C) and ROS (D) are shown, in which 200–300 cells were counted and scored with high intensity of signal.