Supplementation of the medium with testosterone and testosterone precursors reveals that only the 17,20 lyase activity of CYP17 is affected by MEHP treatment.

<p>Effect of 3.5×10⁻⁷ M pregnenolone (+Pregnenolone), 17-OHP (+17-OHP) androstenedione (+Androstenedione) or 10⁻⁷ M testosterone (+Testosterone) in the presence or absence of 10 µM MEHP on 17-OHP, androstenedione, testosterone and 5α-DHT secretion <i>in vitro</i>. Data are presented relative to the corresponding control (Basal). Values are means +/−SEM of 7 testes from fetuses taken from 2 litters in 3 independent experiments. * <i>p<0.05 and ** p<0.01</i> in <i>Wilcoxon Mann-Whitney tests comparing treated and control testes; NS: not significant</i>.