Basal WNT signaling pathway in the CIS-transduced cells.

<p>A) Untransduced NMuMG cells were incubated with 20% Wnt3a- or Wnt5a-containing media for 48 hr. Nuclear protein was extracted and active β-catenin levels were analyzed by immunobloting. Samples were also probed with the anti-lamin B antibody to verify equal loading. B) Nuclear extracts were obtained from NMuMG cells transduced with CIS genes and active β-catenin level was measured by immunobloting. Samples were also probed with the anti-lamin B antibody to verify equal loading. C) mRNA was extracted from NMuMG cells transduced with CIS genes and RT-qPCR was used to measure Ccnd1, Myc, and Tfr1 RNA levels. All values were normalized to GAPDH. Data are the means of three independent experiments with the SE values indicated by error bars. Data were analyzed by Student's t-test. *p≤0.05; **p≤0.01 versus the corresponding control value, as determined by Student's t-test.