3D reconstructions and raw images of NL neurons before and after treatment with ACM.

<p><b>A–D</b>: High frequency neuron with an initial total dendritic length of 428 <i>µ</i>m. Dorsal primary dendritic branches are reduced (−24%) to a single apically projecting branch (white arrowhead). <b>E–H</b>: Middle frequency neuron with an initial total dendritic length of 918 <i>µ</i>m. Several primary dendrites are lost (−33%), both dorsal and ventral (white arrowheads). <b>I–L</b>: Low frequency with an initial total dendritic length of 1351 <i>µ</i>m. Several primary dendrites are lost from the neuron after treatment with ACM (−61%). This effect is most visible on the ventral pole of the neuron (white arrowheads), though also apparent on the dorsal side (white arrows). The changes following treatment with ACM result in a progressive primary dendritic gradient from rostromedial to caudolateral. Scale bars = 15 <i>µ</i>m A–H, K and L; 30 <i>µ</i>m I and J. Dorsal is up in all panels.