<i>iNOS</i> mRNA is induced by LPS, but does not accumulate in <i>Noc</i> KO MEFs.

<p>MEFs treated with LPS for the indicated times were collected for total RNA isolation. mRNA levels of <i>TNFα</i> (<b>A</b>) and <i>iNOS</i> (<b>B</b>) were determined by quantitative RT-PCR and normalized against <i>β2 microglobin</i>. Data represent mean (±SEM) of three independent MEF lines and comparison between genotypes at each time point was done by two-way ANOVA followed by Bonferroni post tests (Prism). N.S. = no significant difference between genotypes (p>0.05).