Akt-GSK3β signaling mediates the keratinocyte migration process.

<p>(A) The inhibitory effects of 30 mM LiCl and 10 µM SH6 on GSK3β and Akt-signaling respectively, were determined by probing the phosphorylated and total forms of GSK3β and Akt. The band intensities were measured and the ratios of phosphorylated/total form were plotted. Bars with the same letter are not significantly different according to the results of one-way ANOVA followed by Duncan’s multiple range tests (<i>p</i><0.05). STS-induced (B) e-lam formation and (C) cell migration were suppressed by LiCl but were induced by SH6. The cell images were captured under 200x and 100x magnification for e-lam formation and cell migration, respectively (<i>top</i>). (D) STS-induced <i>STC1</i> mRNA expression was inhibited by LiCl, but was increased by SH6 (vs STS alone). Asterisks (*) denote <i>p</i><0.05 as compared to their corresponding control.