Results of a dilution series of DNMT3A R882H mutant in a background of wild-type DNA detected by HRMA.

Melt curves and difference plot for MG 67 bp amplicon HRMA.

Jimmy Twin (169618)
Jorgen S. Jensen (169622)
Catriona S. Bradshaw (169624)
Suzanne M. Garland (169628)
Christopher K. Fairley (169632)
Lim Yi Min (169634)
Sepehr N. Tabrizi (169637)

April 2012

A: 23S rRNA gene mutations A2058C (green) and A2059C (yellow). B: 23S rRNA gene mutations A2058G ...

Direct Detection and Quantification of Methylation in Nucleic Acid Sequences using High-Resolution Melting Analysis

Rodriguez Lopez, C.
Asenjo, B.
Lloyd, A.
Wilkinson, M.

January 2010

High-resolution melting (HRM) analysis exploits the reduced thermal stability of DNA fragments that ...

Direct detection and quantification of methylation in nucleic acid sequences using high resolution melting analysis

Lloyd, Amanda J.
Wilkinson, Michael James
Guzmán Asenjo, Beatrice
Rodriguez Lopez, Carlos Marcelino

June 2011

High-resolution melting (HRM) analysis exploits the reduced thermal stability of DNA fragments that ...

Results of a dilution series of DNMT3A R882H mutant in a background of wild-type DNA detected by DNA sequencing.

Jiang Lin (135625)
Dong-ming Yao (135624)
Jun Qian (108736)
Qin Chen (44010)
Wei Qian (56612)
Yun Li (692280)
Jing Yang (13969)
Cui-zhu Wang (135627)
Hai-yan Chai (135630)
Zhen Qian (340706)
Gao-fei Xiao (340709)
Wen-rong Xu (340710)

February 2013

The maximal sensitivity of 10% was obtained. Arrow showed the mutation site.</p

High-Resolution Melting Analysis (HRMA)—More Than Just Sequence Variant Screening

Human Mutationreview

January 2008

ABSTRACT: Transition of the double-stranded DNA molecule to its two single strands, DNA denaturation...

HRMA detects the presence of mutant alleles in mixed populations of wildtype and mutant genomes.

Timothy J. Dahlem (142396)
Kazuyuki Hoshijima (142399)
Michael J. Jurynec (142402)
Derrick Gunther (142406)
Colby G. Starker (134072)
Alexandra S. Locke (142411)
Allison M. Weis (142416)
Daniel F. Voytas (134094)
David Jonah Grunwald (142422)

August 2012

(A) Schematic illustration of the principle of HRMA. A small region of the genome that includes a...

HRM can efficiently detect compound mutations.

Holly R. Thomas (673054)
Stefanie M. Percival (673055)
Bradley K. Yoder (400516)
John M. Parant (673056)

December 2014

A) Depicts compound mutation (different colored cells) in a chimeric animal that could occur due ...

Melt curves and difference plot for MG 147 bp w/unlabelled probe HRMA.

Jimmy Twin (169618)
Jorgen S. Jensen (169622)
Catriona S. Bradshaw (169624)
Suzanne M. Garland (169628)
Christopher K. Fairley (169632)
Lim Yi Min (169634)
Sepehr N. Tabrizi (169637)

April 2012

i. 23S rRNA gene mutation A2058T (red). ii. Wild type (orange). iii. 23S rRNA gene mutation A2059...

Increased DNA methylation at the FXN locus in the 1-month-old YG8sR mouse.

Yogesh K. Chutake (257131)
Whitney N. Costello (798687)
Christina C. Lam (798688)
Aniruddha C. Parikh (798689)
Tamara T. Hughes (798690)
Michael G. Michalopulos (798691)
Mark A. Pook (126056)
Sanjay I. Bidichandani (22466)

September 2015

(A) Normalized melting curves in a high resolution melting (HRM) assay of two reference do...

High resolution melt curves of a mutant DNA sample (gyrA D94G) serially diluted at concentrations of 100%, 50%, 25%, 12.5%, and 6.25%, and mixed with wildtype DNA.

Ann S. G. Lee (332130)
Danny C. T. Ong (332131)
Joshua C. L. Wong (332132)
Gilman K. H. Siu (332133)
Wing-Cheong Yam (332134)

February 2013

Wildtype samples are shown in black and samples with mutations are shown in color. Changes in mel...

HRM can efficiently detect low level chimeric mutations.

Holly R. Thomas (673054)
Stefanie M. Percival (673055)
Bradley K. Yoder (400516)
John M. Parant (673056)

December 2014

A) Depicts different degrees of chimerism (amount red colored cells) that could occur due to cust...

Advances in Nucleic Acid Detection and Quantification 433 Mutation scanning using high-resolution melting

Claire F. Taylor

September 2016

Mutation scanning techniques are used to detect sequence variants without the need for prior knowled...

Representative melting shapes of three R882 mutations and wild-type DNA.

Jiang Lin (135625)
Dong-ming Yao (135624)
Jun Qian (108736)
Qin Chen (44010)
Wei Qian (56612)
Yun Li (692280)
Jing Yang (13969)
Cui-zhu Wang (135627)
Hai-yan Chai (135630)
Zhen Qian (340706)
Gao-fei Xiao (340709)
Wen-rong Xu (340710)

February 2013

WT: wild-type. Three R882 mutations could be easily distinguished according to their different me...

Genotyping of G6PD mutations (G202A and A376G) by high resolution melting (HRM) assay.

Min Lin (17652)
Li Ye Yang (730776)
Dong De Xie (730777)
Jiang Tao Chen (730778)
Santiago-m Monte Nguba (730779)
Carlos Sala Ehapo (730780)
Xiao Fen Zhan (730781)
Juan Urbano Monsuy Eyi (730782)
Rocio Apicante Matesa (730783)
Maximo Miko Ondo Obono (730784)
Hui Yang (91136)
Hui Tian Yang (730785)
Ji Dong Cheng (730786)

April 2015

The arrowhead indicates different genotypes. A and C are temp-shift melting cures; ...

Direct Genotyping of Single Nucleotide Polymorphisms in Methyl Metabolism Genes Using Probe-Free High-Resolution Melting Analysis

Lasse S. Kristensen
Er Dobrovic

September 2016

High-resolution melting (HRM) shows great promise for high-throughput, rapid genotyping of individua...

Melt curves and difference plot for MG 67 bp amplicon HRMA.

Jimmy Twin (169618)
Jorgen S. Jensen (169622)
Catriona S. Bradshaw (169624)
Suzanne M. Garland (169628)
Christopher K. Fairley (169632)
Lim Yi Min (169634)
Sepehr N. Tabrizi (169637)

April 2012

A: 23S rRNA gene mutations A2058C (green) and A2059C (yellow). B: 23S rRNA gene mutations A2058G ...

Direct Detection and Quantification of Methylation in Nucleic Acid Sequences using High-Resolution Melting Analysis

Rodriguez Lopez, C.
Asenjo, B.
Lloyd, A.
Wilkinson, M.

January 2010

High-resolution melting (HRM) analysis exploits the reduced thermal stability of DNA fragments that ...

Direct detection and quantification of methylation in nucleic acid sequences using high resolution melting analysis

Lloyd, Amanda J.
Wilkinson, Michael James
Guzmán Asenjo, Beatrice
Rodriguez Lopez, Carlos Marcelino

June 2011

High-resolution melting (HRM) analysis exploits the reduced thermal stability of DNA fragments that ...

Results of a dilution series of DNMT3A R882H mutant in a background of wild-type DNA detected by DNA sequencing.

Jiang Lin (135625)
Dong-ming Yao (135624)
Jun Qian (108736)
Qin Chen (44010)
Wei Qian (56612)
Yun Li (692280)
Jing Yang (13969)
Cui-zhu Wang (135627)
Hai-yan Chai (135630)
Zhen Qian (340706)
Gao-fei Xiao (340709)
Wen-rong Xu (340710)

February 2013

The maximal sensitivity of 10% was obtained. Arrow showed the mutation site.</p

High-Resolution Melting Analysis (HRMA)—More Than Just Sequence Variant Screening

Human Mutationreview

January 2008

ABSTRACT: Transition of the double-stranded DNA molecule to its two single strands, DNA denaturation...

HRMA detects the presence of mutant alleles in mixed populations of wildtype and mutant genomes.

Timothy J. Dahlem (142396)
Kazuyuki Hoshijima (142399)
Michael J. Jurynec (142402)
Derrick Gunther (142406)
Colby G. Starker (134072)
Alexandra S. Locke (142411)
Allison M. Weis (142416)
Daniel F. Voytas (134094)
David Jonah Grunwald (142422)

August 2012

(A) Schematic illustration of the principle of HRMA. A small region of the genome that includes a...

HRM can efficiently detect compound mutations.

Holly R. Thomas (673054)
Stefanie M. Percival (673055)
Bradley K. Yoder (400516)
John M. Parant (673056)

December 2014

A) Depicts compound mutation (different colored cells) in a chimeric animal that could occur due ...

Melt curves and difference plot for MG 147 bp w/unlabelled probe HRMA.

Jimmy Twin (169618)
Jorgen S. Jensen (169622)
Catriona S. Bradshaw (169624)
Suzanne M. Garland (169628)
Christopher K. Fairley (169632)
Lim Yi Min (169634)
Sepehr N. Tabrizi (169637)

April 2012

i. 23S rRNA gene mutation A2058T (red). ii. Wild type (orange). iii. 23S rRNA gene mutation A2059...

Increased DNA methylation at the FXN locus in the 1-month-old YG8sR mouse.

Yogesh K. Chutake (257131)
Whitney N. Costello (798687)
Christina C. Lam (798688)
Aniruddha C. Parikh (798689)
Tamara T. Hughes (798690)
Michael G. Michalopulos (798691)
Mark A. Pook (126056)
Sanjay I. Bidichandani (22466)

September 2015

(A) Normalized melting curves in a high resolution melting (HRM) assay of two reference do...

High resolution melt curves of a mutant DNA sample (gyrA D94G) serially diluted at concentrations of 100%, 50%, 25%, 12.5%, and 6.25%, and mixed with wildtype DNA.

Ann S. G. Lee (332130)
Danny C. T. Ong (332131)
Joshua C. L. Wong (332132)
Gilman K. H. Siu (332133)
Wing-Cheong Yam (332134)

February 2013

Wildtype samples are shown in black and samples with mutations are shown in color. Changes in mel...

HRM can efficiently detect low level chimeric mutations.

Holly R. Thomas (673054)
Stefanie M. Percival (673055)
Bradley K. Yoder (400516)
John M. Parant (673056)

December 2014

A) Depicts different degrees of chimerism (amount red colored cells) that could occur due to cust...

Advances in Nucleic Acid Detection and Quantification 433 Mutation scanning using high-resolution melting

Claire F. Taylor

September 2016

Mutation scanning techniques are used to detect sequence variants without the need for prior knowled...

Representative melting shapes of three R882 mutations and wild-type DNA.

Jiang Lin (135625)
Dong-ming Yao (135624)
Jun Qian (108736)
Qin Chen (44010)
Wei Qian (56612)
Yun Li (692280)
Jing Yang (13969)
Cui-zhu Wang (135627)
Hai-yan Chai (135630)
Zhen Qian (340706)
Gao-fei Xiao (340709)
Wen-rong Xu (340710)

February 2013

WT: wild-type. Three R882 mutations could be easily distinguished according to their different me...

Genotyping of G6PD mutations (G202A and A376G) by high resolution melting (HRM) assay.

Min Lin (17652)
Li Ye Yang (730776)
Dong De Xie (730777)
Jiang Tao Chen (730778)
Santiago-m Monte Nguba (730779)
Carlos Sala Ehapo (730780)
Xiao Fen Zhan (730781)
Juan Urbano Monsuy Eyi (730782)
Rocio Apicante Matesa (730783)
Maximo Miko Ondo Obono (730784)
Hui Yang (91136)
Hui Tian Yang (730785)
Ji Dong Cheng (730786)

April 2015

The arrowhead indicates different genotypes. A and C are temp-shift melting cures; ...

Direct Genotyping of Single Nucleotide Polymorphisms in Methyl Metabolism Genes Using Probe-Free High-Resolution Melting Analysis

Lasse S. Kristensen
Er Dobrovic

September 2016

High-resolution melting (HRM) shows great promise for high-throughput, rapid genotyping of individua...

Melt curves and difference plot for MG 67 bp amplicon HRMA.

Jimmy Twin (169618)
Jorgen S. Jensen (169622)
Catriona S. Bradshaw (169624)
Suzanne M. Garland (169628)
Christopher K. Fairley (169632)
Lim Yi Min (169634)
Sepehr N. Tabrizi (169637)

April 2012

A: 23S rRNA gene mutations A2058C (green) and A2059C (yellow). B: 23S rRNA gene mutations A2058G ...

Direct Detection and Quantification of Methylation in Nucleic Acid Sequences using High-Resolution Melting Analysis

Rodriguez Lopez, C.
Asenjo, B.
Lloyd, A.
Wilkinson, M.

January 2010

High-resolution melting (HRM) analysis exploits the reduced thermal stability of DNA fragments that ...

Direct detection and quantification of methylation in nucleic acid sequences using high resolution melting analysis

Lloyd, Amanda J.
Wilkinson, Michael James
Guzmán Asenjo, Beatrice
Rodriguez Lopez, Carlos Marcelino

June 2011

High-resolution melting (HRM) analysis exploits the reduced thermal stability of DNA fragments that ...

Results of a dilution series of <i>DNMT3A</i> R882H mutant in a background of wild-type DNA detected by HRMA.

Abstract

Extracted data

Results of a dilution series of <i>DNMT3A</i> R882H mutant in a background of wild-type DNA detected by HRMA.

Abstract

Extracted data

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