<p>Primary (P0) and passage 5 (P5) hAEC expressed HLA-A-B-C but lacked HLA-DP-DQ-DR. Co-stimulatory molecules CD40, CD80 and CD86 remained unaltered at P5. Representative flow plots for HLA antigens and co-stimulatory molecules are shown (<b>A</b>). P0 and P5 hAEC suppressed Concanavelin A stimulated splenocytes from C57/BL6 mice, however P5 cells showed reduced suppression at higher splenocyte ratios (<b>B</b>). P0 and expanded cells produced the immunosuppressive factors TGF-β and IL-6. HLA-G that was abundant in P0 cells declined significantly in P5 grown in DMEM/F12+10%FCS (DF), while hAEC expanded in Epilife lacked HLA-G. Open bars = P0, black shaded bars = P5 cells expanded in DF and grey bars = P5 cells expanded in Epilife. Scale bar...
<p>[A] The data are presented as percentage of Breg cells–IL-10<sup>+</sup>CD5<sup>+</sup>CD1d<sup>+...
Human Leukocyte Antigen (HLA) is a gene that codes for cell-surface proteins that are the basis of o...
<p><b>A</b>, expression of MHC molecules on wt (black lines) and IDE ko (grey lines) NOD splenocytes...
<p>(<b>A</b>) ConA (5 µg/ml) stimulated proliferation of splenocytes from naive mice was dose-depend...
<p>(<b>A</b>) Splenocytes from MOG-immunized and hAEC-treated mice (n = 8) stimulated with MOG pepti...
<p>hAEC∶splenocyte at 1∶40 ratio were co-cultured with splenocytes from EAE mice and MOG (10 µg/ml),...
<p>Bar graphs showing the relative expressions of ICAM-1 (<b>A</b>), HLA-ABC (<b>B</b>), HLA-DR (<b>...
<p>(<b>A</b>) Schematic representation of the procedure for the generation of infected hu-mice: newb...
<p>The hAEC grew well in the xenobiotic-free media Epilife until passage 5 (P5) and plateaued therea...
<p>(A) Flow cytometric analyses were performed to determine the level of human CD45<sup>+</sup> cell...
<p>To determine the HLA-DR and -DQ restriction of Rv2034 p81–100 responding CD4<sup>+</sup> T-cell c...
<p>Adult sublethally irradiated NSG-HLA-A2/HHD mice were transplanted intra-venously with cryoconser...
<p>(A-B) Mice were infected with 2x10<sup><b>7</b></sup> amastigotes intravenously and euthanized at...
<p>(A-C) Analysis of BM and spleen cells from mice with advanced MLL/AF9-OVA leukemia (A) Flow-cytom...
<p>(A) Flow cytometric analysis of HLA-ABC surface expression in hMSCs derived from the BM of three ...
<p>[A] The data are presented as percentage of Breg cells–IL-10<sup>+</sup>CD5<sup>+</sup>CD1d<sup>+...
Human Leukocyte Antigen (HLA) is a gene that codes for cell-surface proteins that are the basis of o...
<p><b>A</b>, expression of MHC molecules on wt (black lines) and IDE ko (grey lines) NOD splenocytes...
<p>(<b>A</b>) ConA (5 µg/ml) stimulated proliferation of splenocytes from naive mice was dose-depend...
<p>(<b>A</b>) Splenocytes from MOG-immunized and hAEC-treated mice (n = 8) stimulated with MOG pepti...
<p>hAEC∶splenocyte at 1∶40 ratio were co-cultured with splenocytes from EAE mice and MOG (10 µg/ml),...
<p>Bar graphs showing the relative expressions of ICAM-1 (<b>A</b>), HLA-ABC (<b>B</b>), HLA-DR (<b>...
<p>(<b>A</b>) Schematic representation of the procedure for the generation of infected hu-mice: newb...
<p>The hAEC grew well in the xenobiotic-free media Epilife until passage 5 (P5) and plateaued therea...
<p>(A) Flow cytometric analyses were performed to determine the level of human CD45<sup>+</sup> cell...
<p>To determine the HLA-DR and -DQ restriction of Rv2034 p81–100 responding CD4<sup>+</sup> T-cell c...
<p>Adult sublethally irradiated NSG-HLA-A2/HHD mice were transplanted intra-venously with cryoconser...
<p>(A-B) Mice were infected with 2x10<sup><b>7</b></sup> amastigotes intravenously and euthanized at...
<p>(A-C) Analysis of BM and spleen cells from mice with advanced MLL/AF9-OVA leukemia (A) Flow-cytom...
<p>(A) Flow cytometric analysis of HLA-ABC surface expression in hMSCs derived from the BM of three ...
<p>[A] The data are presented as percentage of Breg cells–IL-10<sup>+</sup>CD5<sup>+</sup>CD1d<sup>+...
Human Leukocyte Antigen (HLA) is a gene that codes for cell-surface proteins that are the basis of o...
<p><b>A</b>, expression of MHC molecules on wt (black lines) and IDE ko (grey lines) NOD splenocytes...