Morphological characteristic and Immunofluorescence staining of fetal mouse IECs.

<p>(A) The IECs formed a tightly packed monolayer with typical cobblestone morphology (passage 8). (B) HE staining of IECs. (C) Immunofluorescence staining of IEC cytokeratins, cytokeratin 18 was clearly detected in the cytoplasm of IECs with a green color. (D) No green fluorescence in the cytoplasm of IECs was found and only the nucleus was stained red with propidium iodide (PI) in the negative controls.