<p>Panels A, B, C and D: Effect of IVIg and F(ab′)2 fragments on LPS modulation of adhesion molecule expression at the PMN surface. Whole-blood samples were incubated at 37°C for 15 min with PBS, IVIg (1–25 mg/ml) (panel A, C) or equimolar concentrations of F(ab′)<sub>2</sub> (panel B, D) and then with LPS (10 ng/ml) for 30 min. Samples were then stained with PE-anti-human CD11b or anti-L-selectin as described in the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026469#pone-0026469-g001" target="_blank">Figure 1</a>. Results are expressed as mean fluorescence intensity (MFI). Values are means ± sem (n = 3). ° Significantly different from sample incubated with LPS (p<0.05). Panels E and F: Effect of IVIg an...
<p>(<b>A</b>) IgG binding curves against plate-immobilized PFs for IVIg and protein A-purified human...
<p>A: Whole blood was stimulated with LPS (left panels) or PHA (right panel). <i>Upper panels:</i> c...
<p>The quantities of <i>Br</i>-LPS associated to PMNs were determined by Western blotting using a mo...
<p>Whole-blood samples (500 µl) were incubated in 24-well tissue culture plates at 37°C with 5% CO<s...
<p>Whole-blood samples (500 µl) were incubated in 24-well tissue culture plates at 37°C for 20 h wit...
BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading mi...
Background: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading mi...
Background Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading ...
<p>PMNs (2.0×10<sup>6</sup>/ml) were incubated for 45 min at 37°C with <i>E. coli</i> LPS (1 µg/mL) ...
<p>(A) Representative flow cytometry plots detecting CD11b on PMN cells at 6 h after NS or LPS expos...
<p>(A) Heparinized blood was incubated with 100μg/mL of (a) <i>Ec-</i>LPS (corresponding to 7.5 pmol...
<p>NR8383 cells were incubated in fresh culture medium with or without LPS (1 μg/ml). At indicated t...
<p><b>A and B</b>: PMNs (2×10<sup>6</sup>/mL) were stimulated for 45 min with Gal 3 (0.4 µM) and <i>...
<p><b>A</b>: PMNs were primed for 30 min at 37°C with Gal 3 (0.2 µM) or <i>E. coli</i> LPS (1 µg/mL)...
<p>(A–B) The plasma levels of TNF-α and IL-6 were determined using commercial ELISA kits, <i>n</i> =...
<p>(<b>A</b>) IgG binding curves against plate-immobilized PFs for IVIg and protein A-purified human...
<p>A: Whole blood was stimulated with LPS (left panels) or PHA (right panel). <i>Upper panels:</i> c...
<p>The quantities of <i>Br</i>-LPS associated to PMNs were determined by Western blotting using a mo...
<p>Whole-blood samples (500 µl) were incubated in 24-well tissue culture plates at 37°C with 5% CO<s...
<p>Whole-blood samples (500 µl) were incubated in 24-well tissue culture plates at 37°C for 20 h wit...
BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading mi...
Background: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading mi...
Background Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading ...
<p>PMNs (2.0×10<sup>6</sup>/ml) were incubated for 45 min at 37°C with <i>E. coli</i> LPS (1 µg/mL) ...
<p>(A) Representative flow cytometry plots detecting CD11b on PMN cells at 6 h after NS or LPS expos...
<p>(A) Heparinized blood was incubated with 100μg/mL of (a) <i>Ec-</i>LPS (corresponding to 7.5 pmol...
<p>NR8383 cells were incubated in fresh culture medium with or without LPS (1 μg/ml). At indicated t...
<p><b>A and B</b>: PMNs (2×10<sup>6</sup>/mL) were stimulated for 45 min with Gal 3 (0.4 µM) and <i>...
<p><b>A</b>: PMNs were primed for 30 min at 37°C with Gal 3 (0.2 µM) or <i>E. coli</i> LPS (1 µg/mL)...
<p>(A–B) The plasma levels of TNF-α and IL-6 were determined using commercial ELISA kits, <i>n</i> =...
<p>(<b>A</b>) IgG binding curves against plate-immobilized PFs for IVIg and protein A-purified human...
<p>A: Whole blood was stimulated with LPS (left panels) or PHA (right panel). <i>Upper panels:</i> c...
<p>The quantities of <i>Br</i>-LPS associated to PMNs were determined by Western blotting using a mo...