Results of loading on gene expression.

<p>qPCR analysis for SP (<b>a</b>) and NK-1 R (<b>b</b>) mRNA, respectively, in cultured human primary Achilles tendon cells. The mean level of SP mRNA is significantly elevated after 2D-loading of the cultures, whereas the mean level of NK-1 R mRNA is significantly lowered after the loading. Error bars represent standard error of the mean. Level of significance: * p<0.05; **p<0.01 (Independent samples t-test).