Double-labeled cells for orexin-ir and Fos-ir staining.

<p>Schematic drawing (panel A) and photomicrograph (panel B) illustrating the lateral hypothalamus (LH) and cells double-labeled for orexin-ir (brown cytoplasmic staining) and Fos-ir staining in LH in the vole brain. Scale bar = 100 µm in panel B; 10 µm in inset to panel B. Panel C: The males that were re-fed for 2 hr following FD (RF2h) had more orexin-ir cells in the LH than control and food-deprived for 48 hr (FD). Panel D: Both FD and RF2h males had a higher percentage of orexin-ir cells that co-expressed Fos-ir in the LH than the control males. Panel E: Under the food hoarding condition, control males and males that experienced 2 hr food hoarding following FD (RH2h) showed no differences in the number of orexin-ir cells in the LH. Panel F: RH2h males, however, had a higher percentage of orexin-ir cells co-labeled for Fos-ir in the LH than control males. Data are presented as mean ± SEM. * <i>P</i><0.05, ** <i>P</i><0.01, and *** <i>P</i><0.001.