Centrosome number and multinucleation.

<p>NIH-3T3 cells were transfected with (<b>A, C</b>) GFP-aurC-WT, (<b>B, D</b>) GFP-aurC-CA and (<b>E</b>) GFP-aurC-KD vectors, fixed after 96 hours and stained with DAPI and Anti-γ tubulin antibody. (<b>A-B</b>) More than two centrosomes/cell appeared as white dots with anti-γ tubulin staining in (<b>A</b>) GFP-aurC-WT and (<b>B</b>) GFP-aurC-CA transfected cells. (<b>C-D</b>) Multinucleation (more than one nucleus/cell) in (<b>C</b>) GFP-aurC-WT and (<b>D</b>) GFP-aurC-CA in transfected cells. (<b>E</b>) Two centrosomes per cell and only one nucleus/cell in G2 phase of GFP-aurC-KD transfected cells. (<b>F</b>) Histogram shows the percentage of cells with more than 2 centrosomes/cell of 96 hours GFP-aurC-WT, GFP-aurC-CA, GFP-aurC-KD and GFP-alone transfected cells. (<b>G</b>) Histogram shows the percentages of multinucleated cells of 96 hours GFP-aurC-WT, GFP-aurC-CA, GFP-aurC-KD and GFP-alone transfected cells. A minimum of 600 cells was counted for each condition. We performed non-parametric Mann-Whitney test. Results were considered as statistically significant (*) for a p-value under 0.05 when compared to GFP-alone condition.