EPO reverses GD1a/GT1b–2b mAb-mediated inhibition of neurite outgrowth in primary neuronal cultures.

<p>(A) Primary DRG neurons extend long neurites under control conditions. (B) DRG neurons treated with GD1a/GT1b–2b mAb have shorter neurites. (C) EPO reverses Ab-mediated inhibition of neurite outgrowth. Scale bar, 20 µm. (D<b>)</b> EPO induces a dose-responsive reversal of Ab-mediated inhibition of neurite outgrowth in embryonic rat DRG neurons (ER DRG). Quantified data showing that EPO (100 pM) reverses Ab-mediated inhibition of neurite outgrowth in adult mouse DRG (AM DRG), * <i>p</i><0.05 (E), and spinal motor neurons (MN), * <i>p</i><0.01 (F).