Ab reactivities against MAP3865c epitopes are inhibited by the homologous ZnT8 epitopes.

<p>(A) Ab+ and Ab-negative sera from T1D patients were pre-incubated overnight with saturating concentrations of MAP3865c_125–133 (white bars), ZnT8_178–186 (hatched bars), control (grey bars) or no peptide (black bars) and their reactivity on MAP3865c_125–133-coated ELISA plates subsequently tested. (B) The same sera were preincubated with MAP3865c_133–141 (white bars), ZnT8_186–194 (hatched bars), control (grey bars) or no peptide (black bars) and their reactivity on MAP3865c_133–141-coated ELISA plates subsequently tested. Bars depict means ± SEM of triplicate wells and results are representative of two separate experiments.