<p>L cells stably transfected with wild-type and mutant Wnt1 constructs were treated with 100 ug/ml of heparin to release Wnt1 protein bound to HSPGs. After labeling cells with Sulfo-NHS-LC-biotin, cell surface proteins (S) were immunoprecipitated using NeutrAvidin beads. Aliquots of the initial lysates (L; before immunoprecipation) and the immunoprecipitated material were subjected to SDS-PAGE, blotted onto PVDF, and probed with the indicated antibodies. We used Flotillin (cell surface) and ß-tubulin (intracellular) as internal controls. Blots were scanned and quantitated using NIH ImageJ. This experiment was replicated 6 times. Error bars represent the standard error.</p
<p><b>Panel A:</b> L S/L cells were transiently transfected with Wnt1. 4 hrs post-transfection, the ...
<p>A) Intact parental Jurkat T cells, as well as Jurkat T cells stably transfected with full length ...
<p>(A) Growth assay of <i>E. coli</i> BL21 cells expressing Lpa (open triangles) compared to vector ...
<p>HEK293T cells were transiently transfected with constructs encoding either GFP (control), Wnt1-HA...
<p>Stably transfected L cells were grown in the presence (+cycloheximide) and absence (-cycloheximid...
<p><b>Panel A:</b> Mock-transfected L cells were treated with control-conditioned media (Control-CM)...
<p><b>Panel A:</b> L cells stably transfected with vector alone (mock), Wnt1, Wnt1C93A, Wnt1S224A, o...
<p><b>Panel A:</b> Stably transfected L cells were pulsed with <sup>35</sup>S-cys and <sup>35</sup>S...
<p>(A) Representative examples of Western blots used for quantification of DLL1 cell surface present...
<p>Untagged, wild type IFITM1 or IFITM1-Vstop expression plasmids were transfected into HEK293T cell...
<p>A) Right - Western blot detecting the FLAG epitope comparing the amounts of hWNT1, hWNT3A and hWN...
<p>(A) HeLa cell cultures were either mock transfected or transfected with identical amounts of cDNA...
The mouse Wnt-1 gene plays an essential role in fetal brain development and can contribute to tumori...
Wnts are secreted glycolipoproteins fundamental for embryonic development and adult tissue homeostas...
<p>PAE cells expressing <b>A)</b> WT or <b>B)</b> NTKCTK-GlyT1 were incubated with DMSO or PMA (1 μM...
<p><b>Panel A:</b> L S/L cells were transiently transfected with Wnt1. 4 hrs post-transfection, the ...
<p>A) Intact parental Jurkat T cells, as well as Jurkat T cells stably transfected with full length ...
<p>(A) Growth assay of <i>E. coli</i> BL21 cells expressing Lpa (open triangles) compared to vector ...
<p>HEK293T cells were transiently transfected with constructs encoding either GFP (control), Wnt1-HA...
<p>Stably transfected L cells were grown in the presence (+cycloheximide) and absence (-cycloheximid...
<p><b>Panel A:</b> Mock-transfected L cells were treated with control-conditioned media (Control-CM)...
<p><b>Panel A:</b> L cells stably transfected with vector alone (mock), Wnt1, Wnt1C93A, Wnt1S224A, o...
<p><b>Panel A:</b> Stably transfected L cells were pulsed with <sup>35</sup>S-cys and <sup>35</sup>S...
<p>(A) Representative examples of Western blots used for quantification of DLL1 cell surface present...
<p>Untagged, wild type IFITM1 or IFITM1-Vstop expression plasmids were transfected into HEK293T cell...
<p>A) Right - Western blot detecting the FLAG epitope comparing the amounts of hWNT1, hWNT3A and hWN...
<p>(A) HeLa cell cultures were either mock transfected or transfected with identical amounts of cDNA...
The mouse Wnt-1 gene plays an essential role in fetal brain development and can contribute to tumori...
Wnts are secreted glycolipoproteins fundamental for embryonic development and adult tissue homeostas...
<p>PAE cells expressing <b>A)</b> WT or <b>B)</b> NTKCTK-GlyT1 were incubated with DMSO or PMA (1 μM...
<p><b>Panel A:</b> L S/L cells were transiently transfected with Wnt1. 4 hrs post-transfection, the ...
<p>A) Intact parental Jurkat T cells, as well as Jurkat T cells stably transfected with full length ...
<p>(A) Growth assay of <i>E. coli</i> BL21 cells expressing Lpa (open triangles) compared to vector ...