The percentages of circulating Tc17 and Th17 cells in ITP patients and controls.

<p>Heparinized peripheral whole blood from all subjects was stimulated with phorbol myristate acetate, ionomycin, and monensin for 4 h, then stained with labeled antibodies as described in the Design and Methods section. (a) Lymphocytes were gated by flow cytometry in Gate 1. (b,d,f) CD3+ T subsets were gated by flow cytometry; the plots in intern box Gate 2 represent CD3+ T cells. (c, e, g) The percentages of circulating Tc17 and Th17 cells from ITP patients and controls; the percentage of positive cells is shown in each panel.