Alterations in endogenous H₂S synthesis 14 days after doxorubicin injection (A–D) and effect of S-diclofenac on the synthesis of H₂S in doxorubicin-induced cardiomyopathy (C, D).

<p>Male 10-week-old C57BL/6J mice were randomly given doxorubicin (15 mg/kg, i.p.) or same volume of saline. Treatment with S-diclofenac (25 and 50 µmol/kg, i.p.) or diclofenac (25 and 50 µmol/kg, i.p.) started 2 hours after doxorubicin injection and continued every 24 hours. Fourteen days after doxorubicin injection, the expression level of CSE mRNA (A) and protein in hearts (B), H₂S synthesizing activity in hearts (C) and plasma H₂S concentration (D) were measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026441#s4" target="_blank">Materials and Methods</a>. Results shown are the mean ± SEM (n = 6–8 animals in each group). * P<0.01 vs saline group; † P<0.05 vs Dox+Diclo 25 µmol/kg group (animals treated with doxorubicin+diclofenac 25 µmol/kg); ‡ P<0.01 vs Dox+Diclo 50 µmol/kg group (animals treated with doxorubicin+diclofenac 50 µmol/kg).