CD4 T cell help is not required for long-lived protection against B16 tumor challenge.

<p>Mice were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026491#pone-0026491-g004" target="_blank">Figure 4A</a>. Sixty days post-surgery, mice were stratified based on the development of vitiligo. Vitiligo-affected hosts and unaffected hosts that received either Transient Help or No Help, or naïve mice (No Treatment), were challenged with B16 melanoma cells, and tumor incidence was followed. Comparisons are indicated by brackets, and statistical significance was determined by Log-rank analysis, with *** <i>P</i><0.0002 and <i>NS</i> denoting <i>P</i>>0.05. Data are combined from 3 experiments, with a total of 8–12 mice per group.