Phenethyl isothiocyanate (PEITC) increases nuclear levels of cleaved Notch2 protein in LNCaP and PC-3 cells.

<p>(A) Immunofluorescence microscopic images depicting nuclear levels of cleaved Notch2 protein in LNCaP and PC-3 cells after 8-hour treatment with dimethyl sulfoxide (DMSO) or 5 µM PEITC at 100× objective magnification. Quantitation of <i>Notch1</i>, <i>Notch2</i>, <i>Jagged1</i>, and <i>Jagged2</i> mRNA levels by real-time RT-PCR in (B) LNCaP and (C) PC-3 cells after 8-hour treatment with DMSO (control) or PEITC (2.5 or 5 µM). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026615#s2" target="_blank">Results</a> shown are mean ± SD (n = 3). *Significantly different (<i>P</i><0.05) compared with DMSO-treated control by one-way ANOVA with Dunnett's adjustment. Each experiment was repeated at least twice.