Expression of glucocorticoid, mineralocorticoid and thyroid hormone receptors in the lateral and basolateral nuclei of the amygdala in euthyroid and hypothyroid rats.

<p>(<b>A</b>) Amygdala volume estimation in E and H rats. No differences were found between groups. Representative photomicrographs (40×) of MR (<b>B, C</b>) and GR (<b>E, F</b>) immunohistochemistry in LA/BLA in E (Eu) and H (Hypo) animals. Scale bar, 20 µm. Histograms show the mean gray values of GR (<b>D</b>) and MR (<b>G</b>) obtained for E and H animals (n = 4 per group). GR and MR staining were increased in H rats. Panoramic views of isotopic <i>in situ</i> hybridization for TRα1 (<b>H, I</b>) and TRβ1 (<b>K, L</b>) in coronal sections of the brain of E (Eu) and H (Hypo) animals. Scale bar, 0.15 cm. Arrows point to the amygdala. Histograms show the mean gray values in arbitrary units (AU) obtained for E and H rats for TRα1 (<b>J</b>) and TRβ1 (<b>M</b>) mRNA expression (n = 3 per group). No differences were found between groups for TRα1. TRβ1 expression was reduced in H rats. Plot bars show means ± SEM. *, p<0.05; **, p<0.01; NS, no significant.