Overexpression of zM6Ab can induce neurite outgrowth and filopodia in PC12 and COS-1 cells.

<p>(A) PC12 cells were transfected with the control pcDNA3-GFP or pcDNA3-M6Ab-GFP or pcDNA3-M6Ab-T166A-GFP plasmids. Twenty-four hours after transfection, cells were treated with nerve growth factor (NGF) (100 ng/ml) for 2 days. Cells were then fixed, and images were taken with a Zeiss LSM510 laser scanning confocal microscope. The insets are the 2× magnified images of the boxed areas. (B) Quantification of the total number of neurites, total length of neurites, and filopodium-like processes in a 20-µm neurite length. * indicates a significant difference compared with the respective control of GFP (P<0.05). (C) COS-1 cells were transfected with the control pcDNA3-GFP or pcDNA-M6Ab-GFP or pcDNA3-M6Ab-T166A-GFP plasmids. Cells were fixed, and images were taken with a Zeiss LSM510 laser scanning confocal microscope. Scale bars, 10 µm.