Chronic infection reduces IL-7Rα^hi memory cells in both memory subsets.

<p>Mice were infected with 10⁵<i>P. chabaudi</i>. Days 30–34 half of the mice were treated with chloroquine (<b>+CQ</b>), while the other mice retained a chronic infection (<b>-CQ</b>). 2.5 months after infection, splenocytes were analyzed by flow cytometry for CD4, CD44, CD62L and (<b>A, B</b>) IL-7Rα (CD127) or incorporation of BrdU dosed into the water days 62–72 as an indicator of homeostatic proliferation, (<b>C, D</b>). Naïve cells (CD44^lo) were used as an internal control to set the quadrants (<b>B</b>, left). Dotted lines represent chloroquine treated mice (+CQ) while bold lines represent chronic infection (-CQ). Data shown is the average of 4–5 mice per group and experiment was repeated twice with similar results. Contour plots (10% with outliers) are gated as described on each plot and are from representative animals. <b>*</b> indicates p≤0.05, <b>**</b> p≤0.01.