Impact of the two isogenic groups of pediatric <i>H. pylori</i> strains on the NCI-N87 cells morphology.

<p>NCI-N87 cells grown to 80–90% confluence were co-cultured for 24 h with the pool of five isogenic NUD-associated <i>H. pylori</i> strains (NUD) and in parallel with the pool of five isogenic PUD strains (PUD). The control corresponds to non-infected NCI-N87 cells. A), B), and C) light microscopy observations. D), E), and F) immunodetection of the microtubule network (green) (1∶1000 α-tubulin antibody plus a FITC conjugated secondary antibody). G), H), and I) PAS staining alone, and in J), L), and M) in conjugation with hematoxylin. Dark arrow indicates extracellular vesicles that reacted with PAS.