<p>Lower case letters indicate phosphorothioated bonds and underlined nucleotides in the reverse primers highlight the NNK degenerated codons.</p
<p>DNAzymes, RNA substrates and PCR primers for reverse transcription. Underlined letters denote ‘10...
<p>Sequence of oligonucleotides used to construct plasmids and yeast strains.</p
a<p>Lower case base pairs target recombination and upper case base pairs direct amplification.</p
<p>(A) Selection of targeted codons for NNK saturation (dark-grey highlighted letters). (B) Twelve n...
a<p>: Nucleotides underlined correspond to the codons chosen for mutation. Nucleotides in parenthese...
<p>Emboldened sequences represent degenerate codons (N = A+C+G+T, K = G+T, M = A+C). Underlined sequ...
<p>PHO indicates 5′ phosphate modification. Underlined sequences represent degenerate codon (N = A+C...
*<p>Corresponding amino acid sequences for degenerate primers were shown in parentheses.</p
<p><sup>a</sup>Underlined sequence indicates restriction site.</p><p><sup>b</sup>Bold sequence indic...
<p>Nucleotides altered by site directed mutagenesis are underlined.</p><p>Oligonucleotides used for ...
<p>Oligomers sequences. PNA bases are indicated with lower case letters, DNA bases with upper case l...
<p>Underlined and bold nucleotides indicate the nucleotides used to replace the wild-type amino acid...
<p>Oligonucleotides used for the construction of the GFP fused truncated N proteins.</p
<p>O: orientation indicated as S (sense) and AS (antisense); Capital letters denote regions overlapp...
<p>Oligonucleotide sequences of primers and annealing temperatures used for this study.</p
<p>DNAzymes, RNA substrates and PCR primers for reverse transcription. Underlined letters denote ‘10...
<p>Sequence of oligonucleotides used to construct plasmids and yeast strains.</p
a<p>Lower case base pairs target recombination and upper case base pairs direct amplification.</p
<p>(A) Selection of targeted codons for NNK saturation (dark-grey highlighted letters). (B) Twelve n...
a<p>: Nucleotides underlined correspond to the codons chosen for mutation. Nucleotides in parenthese...
<p>Emboldened sequences represent degenerate codons (N = A+C+G+T, K = G+T, M = A+C). Underlined sequ...
<p>PHO indicates 5′ phosphate modification. Underlined sequences represent degenerate codon (N = A+C...
*<p>Corresponding amino acid sequences for degenerate primers were shown in parentheses.</p
<p><sup>a</sup>Underlined sequence indicates restriction site.</p><p><sup>b</sup>Bold sequence indic...
<p>Nucleotides altered by site directed mutagenesis are underlined.</p><p>Oligonucleotides used for ...
<p>Oligomers sequences. PNA bases are indicated with lower case letters, DNA bases with upper case l...
<p>Underlined and bold nucleotides indicate the nucleotides used to replace the wild-type amino acid...
<p>Oligonucleotides used for the construction of the GFP fused truncated N proteins.</p
<p>O: orientation indicated as S (sense) and AS (antisense); Capital letters denote regions overlapp...
<p>Oligonucleotide sequences of primers and annealing temperatures used for this study.</p
<p>DNAzymes, RNA substrates and PCR primers for reverse transcription. Underlined letters denote ‘10...
<p>Sequence of oligonucleotides used to construct plasmids and yeast strains.</p
a<p>Lower case base pairs target recombination and upper case base pairs direct amplification.</p
DOI
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