Acute β2AR stimulation induced increases in phosphorylation of P70S6, S6, GSK3α, GSK3β and FOXO3a.

<p>C57BL/6 mice were treated with vehicle control, dobutamine (Dob., 1.7 mg/kg, i.p.), or formoterol (For., 2.1 mg/kg, i.p.), for 30 min. Shown are representative Western blots performed on left ventricular lysates with antibodies against phospho-P70S6K (Thr389), phospho-P70S6K (Thr421/Ser424), phospho-S6 (Ser235/236), phospho-S6 (Ser240/244), phospho-GSK-3α (Ser21), phospho-GSK-3β (Ser9), phospho-FOXO3a (Ser318/321). Blots of individual total protein and activ were also included.