1D ¹H (A, D), 2D ¹H-¹³C HMQC (F, G), ¹H-¹H HOHOHA τ_m 110 ms (E) and ¹H-¹H NOESY τ_m 500 ms (B, C, H) spectra of ShLTA.

<p>¹H (D), ¹H-¹³C HMQC, ¹H-¹H HOHOHA and ¹H-¹H NOESY (H) were recorded in D₂O, ¹H (A) and ¹H-¹H NOESY (B, C) in H₂O/D₂O (9∶1) at 298K. Expanded regions (δ¹H: 8.10–8.40) (A), (δ¹H: 8.10–8.40, δ¹H: 2.00–2.30) (B), (δ¹H: 8.10–8.40, δ¹H: 3.20–5.70) (C), (δ¹H: 5.05–5.50) (D), (δ¹H: 5.05–5.50, δ¹H: 3.20–5.70) (E), (δ¹H: 5.05–5.50, δ¹³C: 95–100) (F), (δ¹H: 3.90–4.40, δ¹³C: 65–80) (G) and (δ¹H: 3.90–4.40, δ¹H: 3.20–5.70) (H) are shown. GI, α-GlcN, GII, α-GlcNAc, gI to gIV, Gro units (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026316#pone-0026316-t003" target="_blank">Table 3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026316#pone-0026316-g004" target="_blank">Figure 4</a>). GIII corresponds to a weakly abundant GlcNAc that could not be further characterized.