SiRNA-directed knockdown of Osx impairs VDR gene expression in MC3T3 osteoblasts.

<p>(A) RNA expression levels as determined by quantitative real-time RT-PCR. MC3T3 osteoblasts were transfected with siRNA targeting mouse Osx. RNA was isolated 24 h post-transfection and quantitated by real-time RT-PCR. The RNA level from the control siRNA group was normalized to a value of 1. Values were presented as the mean ±S.D. A paired <i>t</i>-test was performed comparing si-control group and si-Osx group. (B) Western analysis of the Osx knockdown. Protein was isolated by acetone precipitation of whole cell lysates and then analyzed by western blot using rabbit anti-VDR or anti-Osx polyclonal antibodies. Beta-actin was used as a loading control.