Characteristics induced by 2 weeks exposure of SRA01/04 cells to low-doses of H₂O₂.

<p>A. Shows that cell viability (Left) of SRA01/04 cells decreases with increasing concentrations of H₂O₂ as measured by the MTT assay (Mean± SD; n = 5.). Morphologic changes (Right) of SRA01/04 cells after 2 weeks exposure to 50 µM H₂O₂ demonstrate gross enlargement, flattening, and the accumulation of granular cytoplasmic inclusions (Arrows indicate the nuclei of typical senescent cells, 100×). B. Shows staining for SA-β-gal activity increased in SRA01/04 cells exposed to 50 µM H₂O₂ for 2 weeks expressed as either SA-β-gal-positive cell index (Mean± SD; n = 3. * <i>P</i><0.05. Left) or actual cellular staining (Right). C. Shows that DNA synthesis decreased in SRA01/04 cells exposed to 50 µM H₂O₂ for 2 weeks. BrdU labeling in SRA01/04 cells expressed either by BrdU incorporation and DAPI cell viability labeling (Mean± SD; n = 3. * <i>P</i><0.05. Left) or expression of BrdU labeling of cells (Right). D. Shows that Ndrg2 protein is up-regulated in SRA01/04 cells exposed to 0–50 µM H₂O₂ for 2 weeks (Mean± SD; n = 3. * <i>P</i><0.05 compare with normal SRA01/04 cells).